Survival of Rice Non-embryogenic Callus Cells after Cryopreservation in Liquid Nitrogen by Vitrification.
نویسندگان
چکیده
منابع مشابه
Ice-Free Cryopreservation by Vitrification
Long-term preservation is crucial as an enabling technology for tissue banking for transplantation, regenerative medicine products that contain living cells and biopsy samples. Regenerative medicine reviews in particular have consistently highlighted the need for preservation methods for product development and commercialization. Strategic assessments of regenerative medicine have provided thre...
متن کاملCryopreservation of human granulocytes in liquid nitrogen.
Human granulocytes (PMNL) were successfully cryopreserved for up to 14 months. The PMNL (1-2 X 10(7)/ml) were stored in 2-ml ampoules in the gas phase of liquid nitrogen at a temperature between -160 degrees C and -196 degrees C using dimethylsulphoxide (DMSO 10%) as cryoprotectant. Morphology and phagocytic and bactericidal capacity were best preserved by adding fetal calf serum to the freezin...
متن کاملSimple vitrification protocol for cryopreservation of oil palm using embryogenic culture
Friable embryogenic tissue (FET) of oil palm was successfully cryopreserved by vitrification. After preconditioning the tissue on Murashige and Skoog (MS) medium supplemented with 0.25 M sucrose for 7 days, FET was dehydrated for 60 min at 0°C with a highly concentrated vitrification solution (PVS2) and plunged directly into liquid nitrogen gave the highest percentage of somatic embryo (SE) for...
متن کاملBiochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.
Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...
متن کاملLabel-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis
The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 1998
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.15.35